This case presentation illustrates the diagnosis, treatment, and monitoring of a multidrug-resistant hookworm (Ancylostoma caninum) infection in a Greyhound dog using an experimental treatment protocol. The patient, a 2.5-year-old male castrated (MC) Greyhound, presented with a hookworm diagnosis and treatment history that included praziquantel, pyrantel pamoate, febantel, and imidacloprid/moxidectin. His fecal egg count (FEC) was 55 eggs per gram.